Journal: International Journal of Nanomedicine

Article Title: Drug Delivery to the Bone Microenvironment Mediated by Exosomes: An Axiom or Enigma

doi: 10.2147/IJN.S307843

Figure Lengend Snippet: Patents Related to the Diagnostic and Therapeutic Application of Exosomes Towards Bone Disorders

Article Snippet: Bone marrow-derived mesenchymal stem cells (BM-MSCs) and Adipose-derived stem cells (ASCs) , WO2019139762A1 , Zen-Bio, Inc. (US) , Exosome compositions and use thereof for joint disorders and diseases , Filed on 2018/Status pending.

Techniques: Diagnostic Assay, Biomarker Discovery, Derivative Assay, CRISPR, Isolation, Injection, Clinical Proteomics

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Nanoparticle tracking analysis and EV morphology. NTA was performed on two biological replicates (i.e., Lot 1 and Lot 2) of ( a ) hTERT-immortalized MSC EVs and ( b ) PC3 EVs to analyze relative concentration (particles/mL) and size distribution. Dashed lines indicate values of 50 nm and 200 nm. ( c ) The average % of EVs from each lot that measured between 50–200 nm in size and 200+ nm in size. n = 6 (2 biological replicates and each biological replicate was measured in triplicate). ** = p < 0.01. Representative TEM images show the appearance of ( d ) hTERT-immortalized MSC EVs and ( e ) PC 3 EVs. Scale bar = 100 nm.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXM™), PC3 (ATCC ® CRL-1435-EXM™), and HCT 116 (ATCC ® CCL-247™).

Techniques: Concentration Assay

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Characterization of EVs. ( a ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. ( b ) Western blot for CD63, CD9, CD81, and GAPDH from two independent lots (biological replicates) of PC3 EVs. Multiplex analysis was performed to analyze tetraspanins (CD63, CD81, and CD9). ( c ) Average ECL values from two independent lots (biological replicates) of hTERT-immortalized MSC EVs. Each lot was assayed in duplicate. ( d ) Average ECL values from two independent lots (biological replicates) of PC3 EVs. Each lot was assayed in duplicate. (* p < 0.05; ** p < 0.01; **** p < 0.0001).

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXM™), PC3 (ATCC ® CRL-1435-EXM™), and HCT 116 (ATCC ® CCL-247™).

Techniques: Western Blot, Multiplex Assay

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: EV surface marker profiling and cell migration Assay. Multiplex analysis was performed to analyze various EV-associated surface marker proteins. Assays were run in duplicate. The average ECL values of each surface marker were analyzed and compared between hTERT-immortalized MSC and PC3 EVs. ( a ) THY-1, Thrombomodulin, and Neprilysin were significantly enriched in hTERT-immortalized MSC EVs. ( b ) MCAM, EPCAM, ICAM-1, EGFR, and ALCAM were significantly enriched in PC3 EVs. **** p < 0.0001; *** p < 0.001; ** p < 0.01. ( c ) A cell migration assay was performed using RPE cells. After the creation of an artificial gap, cells were immediately treated with EVs at an approximate ratio of 1:10,000 (recipient cell:EV). Representative images display gap closure over 48 h in response EVs. Scale bar = 1000 µm. ( d ) GraphPad Prism was used to analyze raw images to quantify percent gap coverage. **** p < 0.0001 relative to untreated.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXM™), PC3 (ATCC ® CRL-1435-EXM™), and HCT 116 (ATCC ® CCL-247™).

Techniques: Marker, Cell Migration Assay, Multiplex Assay

Journal: Cells

Article Title: hTERT-Immortalized Mesenchymal Stem Cell-Derived Extracellular Vesicles: Large-Scale Manufacturing, Cargo Profiling, and Functional Effects in Retinal Epithelial Cells

doi: 10.3390/cells13100861

Figure Lengend Snippet: Profiling of EV-associated cargo. ( a ) The results from MS show the number of unique and shared proteins between hTERT-immortalized MSC EVs and PC3 EVs. ( b ) The STRING database was used to calculate the PPIs of peptides extracted from each EV preparation. Proteins are represented by nodes that are connected by lines representative of their confidence level. Colors represent specific functions that are associated with proteins. ( c ) Total RNA was isolated from hTERT-immortalized MSCs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( d ) Total RNA was isolated from hTERT-immortalized MSC EVs, and RT-qPCR was performed to target candidate mRNAs. Average Cq values are shown. n = 3. ( e ) EVs were assayed for the presence of inflammatory cytokines using the S-PLEX ® proinflammatory Panel 1 Kit (Meso Scale Diagnostics) following the manufacturer’s instructions. Assays were run in duplicate. Analyte concentrations were calculated using a 4-PL fit of a standard curve of the calibrators. The data shown represent samples assayed without lysis. *** p < 0.001; * p < 0.05.

Article Snippet: The EVs used in this study are available from ATCC as follows: hTERT -immortalized MSC (ATCC ® SCRC-4000-EXM™), PC3 (ATCC ® CRL-1435-EXM™), and HCT 116 (ATCC ® CCL-247™).

Techniques: Isolation, Quantitative RT-PCR, Lysis

Journal: Stem Cell Research & Therapy

Article Title: Human-induced pluripotent stem cell derived exosomal miR-103a-3p accelerate urethral injury recovery by promoting angiogenesis via TGFBR3/VEGF/FAK axis

doi: 10.1186/s13287-026-04939-0

Figure Lengend Snippet: Characterization of hiPSC-Exo. A Transmission electron microscopy image of hiPSC-Exo. B Particle size distribution of hiPSC-Exo detected by nanoparticle tracking analysis (NTA). C Western blot analysis of hiPSC-Exo for the specific markers Calnexin, TSG101 and CD9. D PKH26 analysis of hiPSC-Exo uptake by HUVEC (red: PKH26 labeled hiPSC-Exo; green, phalloidin; blue, DAPI)

Article Snippet: This study indicated that hiPSC-Exo played a crucial role in promoting angiogenesis to accelerate urethral injury repair through the action of miR-103a-3p in exosome on the TGFBR3/VEGF/FAK signaling pathway in endothelial cells.

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Labeling

Journal: Stem Cell Research & Therapy

Article Title: Human-induced pluripotent stem cell derived exosomal miR-103a-3p accelerate urethral injury recovery by promoting angiogenesis via TGFBR3/VEGF/FAK axis

doi: 10.1186/s13287-026-04939-0

Figure Lengend Snippet: HiPSC-Exo promotes angiogenesis in mouse lower limb ischemia model and rat urethral injury recovery model. A Laser scatter flow imaging of lower limb ischemia situation on postoperative days 0, 7, 14, and 21 between sham group, model group and hiPSC-Exo group. B Urethrography imaging of urethral injury recovery on postoperative days 7 between model group and hiPSC-Exo group. C HE staining of the urethral repair on postoperative days 7. D Microscopic images of two groups stained with vascular marker CD31 and proliferation marker PCNA in rat urethral injury recovery. * p < 0.05, *** p < 0.001

Article Snippet: This study indicated that hiPSC-Exo played a crucial role in promoting angiogenesis to accelerate urethral injury repair through the action of miR-103a-3p in exosome on the TGFBR3/VEGF/FAK signaling pathway in endothelial cells.

Techniques: Imaging, Staining, Marker

Journal: Stem Cell Research & Therapy

Article Title: Human-induced pluripotent stem cell derived exosomal miR-103a-3p accelerate urethral injury recovery by promoting angiogenesis via TGFBR3/VEGF/FAK axis

doi: 10.1186/s13287-026-04939-0

Figure Lengend Snippet: hiPSC-Exo regulates proliferation, migration and angiogenesis of HUVEC. A Representative images of wound healing of HUVEC in control group and hiPSC-Exo group after 24 h. B CCK-8 assay to assess the proliferative ability of HUVEC in control group and hiPSC-Exo group at different time. C Statistical analysis of wound closure rate. D Representative images of migrative ability of HUVEC in control group and hiPSC-Exo group after 24 h. E Statistical analysis of migratory cells. F Representative images of tube formation of HUVEC in control group and hiPSC-Exo group after 6 h. G Statistical analysis of total tubule length and junction node. H Western blot analysis of VEGF expression in control group and hiPSC-Exo group. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: This study indicated that hiPSC-Exo played a crucial role in promoting angiogenesis to accelerate urethral injury repair through the action of miR-103a-3p in exosome on the TGFBR3/VEGF/FAK signaling pathway in endothelial cells.

Techniques: Migration, Control, CCK-8 Assay, Western Blot, Expressing

Journal: Stem Cell Research & Therapy

Article Title: Human-induced pluripotent stem cell derived exosomal miR-103a-3p accelerate urethral injury recovery by promoting angiogenesis via TGFBR3/VEGF/FAK axis

doi: 10.1186/s13287-026-04939-0

Figure Lengend Snippet: hiPSC-Exo enhance angiogenesis through up-regulating miR-103a-3p. A Heatmap showing miRNA microarray results of hiPSC-Exo. B qPCR validation of the expression of the differential genes in micoRNA-seq. C Representative images captured the uptake of miR-103a-3p mimic labeled by cy3 in hiPSC-Exo by HUVEC after 24 h. * p < 0.05, ** p < 0.01

Article Snippet: This study indicated that hiPSC-Exo played a crucial role in promoting angiogenesis to accelerate urethral injury repair through the action of miR-103a-3p in exosome on the TGFBR3/VEGF/FAK signaling pathway in endothelial cells.

Techniques: Microarray, Biomarker Discovery, Expressing, Labeling